mouse il 10 elisa kit (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
96
Structured Review
R&D Systems
mouse il 10 elisa kit
![circSMAD4 drives tumor-educated M2-like polarization of macrophages and promotes tumor-cell aggressiveness. (A) Workflow for generating TC-hMDMs and TC-BMDMs, circSMAD4 knockdown, and downstream functional assays. (B) RT–qPCR analysis of M1-associated markers (MHC-II [HLA-DRA in TC-hMDMs; H2-Ab1 in TC-BMDMs], NOS2, and CD86) and M2-associated markers (CD163, CD206, and ARG1) in TC-hMDMs and TC-BMDMs. (C) Representative flow-cytometry histograms for HLA-DR, iNOS, CD86, CD163, CD206, and ARG1 in TC-hMDMs. Gating strategy and marker thresholds were defined based on FMO controls (see ). (D) Flow-cytometry quantification of marker-positive cells in TC-hMDMs and TC-BMDMs. (E) ELISA of <t>IL-10,</t> TGF-β, and iNOS in culture supernatants. (F) CCK-8 assays of A549 and LLC cells. (G) Colony-formation assays of A549 and LLC cells with quantification. (H) Bioluminescence-based growth readouts of patient-derived LUAD organoids (PDO #1 and PDO #2) after co-culture with TC-hMDMs. (I) Immunoblot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin) in A549 and LLC cells. (J) Transwell migration and invasion assays of A549 and LLC cells with quantification. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0104/pmc13050104/pmc13050104__gr2.jpg)
Mouse Il 10 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+10/Mouse+IL-10+Quantikine+ELISA+Kit/pmc13050104-63-9-13
Average 96 stars, based on 398 article reviews
Mouse Il 10 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+10/Mouse+IL-10+Quantikine+ELISA+Kit/pmc13050104-63-9-13
Average 96 stars, based on 398 article reviews
mouse il 10 elisa kit - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma"
Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
Journal: Non-coding RNA Research
doi: 10.1016/j.ncrna.2026.03.003
Figure Legend Snippet: circSMAD4 drives tumor-educated M2-like polarization of macrophages and promotes tumor-cell aggressiveness. (A) Workflow for generating TC-hMDMs and TC-BMDMs, circSMAD4 knockdown, and downstream functional assays. (B) RT–qPCR analysis of M1-associated markers (MHC-II [HLA-DRA in TC-hMDMs; H2-Ab1 in TC-BMDMs], NOS2, and CD86) and M2-associated markers (CD163, CD206, and ARG1) in TC-hMDMs and TC-BMDMs. (C) Representative flow-cytometry histograms for HLA-DR, iNOS, CD86, CD163, CD206, and ARG1 in TC-hMDMs. Gating strategy and marker thresholds were defined based on FMO controls (see ). (D) Flow-cytometry quantification of marker-positive cells in TC-hMDMs and TC-BMDMs. (E) ELISA of IL-10, TGF-β, and iNOS in culture supernatants. (F) CCK-8 assays of A549 and LLC cells. (G) Colony-formation assays of A549 and LLC cells with quantification. (H) Bioluminescence-based growth readouts of patient-derived LUAD organoids (PDO #1 and PDO #2) after co-culture with TC-hMDMs. (I) Immunoblot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin) in A549 and LLC cells. (J) Transwell migration and invasion assays of A549 and LLC cells with quantification. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.
Techniques Used: Knockdown, Functional Assay, Quantitative RT-PCR, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Derivative Assay, Co-Culture Assay, Western Blot, Migration
Related Articles
Enzyme-linked Immunosorbent Assay:Article Title: Lycium barbarum polysaccharides and/or Bifidobacterium longum exert anti-inflammation and modulate gut microbiota in rats with dextran sulfate sodium-induced ulcerative colitis. Article Snippet: Ulcerative colitis (UC) is a bowel disease with intestinal inflammation.. This study investigated the effects of Lycium barbarum polysaccharides (LBP) and/or Bifidobacterium longum OLP-01 (OLP-01) in rats with dextran sulfate sodium (DSS)-induced UC.. Rats were divided into 5 groups: normal, DSS-induced UC, UC treated with LBP (100 mg/kg bw), UC treated with OLP-01 (2 × 109 CFU/kg bw), and UC treated with combined LBP (50 mg/ kg bw) and OLP-01 (1 × 109 CFU/kg bw) groups. Article Title: Repositioning of Ertiprotafib via supramolecular assembly enables biomarker-guided therapy for acute kidney injury. Article Snippet: .. Supernatants derived from homogenized kidney tissue were examined for inflammatory cytokines—TNF- (43907, BioLegend), IL-1 (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems) and Article Title: Regular Cold‐Water Immersion Following HIIT Does Not Affect Intramuscular Adaptation Markers, Inflammatory Profile or Endurance Performance Article Snippet: .. To evaluate muscle inflammatory status, approximately 50 mg of wet muscle tissue was homogenized in extraction buffer, and the concentrations of IL‐6 (DY206; assay range: 78.1–5000 pg mL −1 ), Article Title: Particle dissolution rate controls macrophage response and drug release from mesoporous silica inhalation carriers. Article Snippet: .. Inflammatory responses of THP-1–derived and primary human macrophages were assessed by measuring the secretion of TNF-α, Derivative Assay:Article Title: Repositioning of Ertiprotafib via supramolecular assembly enables biomarker-guided therapy for acute kidney injury. Article Snippet: .. Supernatants derived from homogenized kidney tissue were examined for inflammatory cytokines—TNF- (43907, BioLegend), IL-1 (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems) and Expressing:Article Title: Targeting MAFB potentiates immune checkpoint inhibitor efficacy by reprogramming tumor-associated macrophages to an M1-like phenotype in colorectal cancer. Article Snippet: Colorectal cancer (CRC) remains largely resistant to immune checkpoint inhibitors (ICIs) due to an immunosuppressive tumor microenvironment (TME) shaped by M2-like tumor-associated macrophages (TAMs).. Identifying transcriptional regulators of M2-like TAMs in CRC could provide strategies to overcome ICI resistance by reprogramming the TME.. In this study, we analyzed single-cell RNA-seq data from CRC patients to identify transcriptional regulators of M2-like TAMs. Incubation:Article Title: Targeting MAFB potentiates immune checkpoint inhibitor efficacy by reprogramming tumor-associated macrophages to an M1-like phenotype in colorectal cancer. Article Snippet: Colorectal cancer (CRC) remains largely resistant to immune checkpoint inhibitors (ICIs) due to an immunosuppressive tumor microenvironment (TME) shaped by M2-like tumor-associated macrophages (TAMs).. Identifying transcriptional regulators of M2-like TAMs in CRC could provide strategies to overcome ICI resistance by reprogramming the TME.. In this study, we analyzed single-cell RNA-seq data from CRC patients to identify transcriptional regulators of M2-like TAMs. Article Title: Study on the role and mechanism of the IL-10-IRF-8 signaling pathway in bone marrow mesenchymal stem cell therapy for pain in CCD rats Article Snippet: .. And then transferred to membranes, blocked, and incubated with primary antibodies against β-actin (1:3000, Affinity, AF7018, England), Arg-1 (1:10000, Proteintech, 66129-1-Ig, USA), Iba-1 (1:5000, HUABIO, ET1705-78, China), IRF-8 (1:500, Affinity, DF13627, England), and Article Title: EXPRESS: Study on the Role and Mechanism of the IL-10-IRF-8 Signaling Pathway in Bone Marrow Mesenchymal Stem Cell Therapy for Pain in CCD Rats. Article Snippet: .. And then transferred to membranes, blocked, and incubated with primary antibodies against β-actin (1:3000, Affinity, AF7018, England), Arg-1 (1:10000, Proteintech, 66129-1-Ig, USA), Iba-1 (1:5000, HUABIO, ET1705-78, China), IRF-8 (1:500, Affinity, DF13627, England), and Extraction:Article Title: Regular Cold‐Water Immersion Following HIIT Does Not Affect Intramuscular Adaptation Markers, Inflammatory Profile or Endurance Performance Article Snippet: .. To evaluate muscle inflammatory status, approximately 50 mg of wet muscle tissue was homogenized in extraction buffer, and the concentrations of IL‐6 (DY206; assay range: 78.1–5000 pg mL −1 ), |
